国产精品美女久久久久久,欧美操逼123区,欧美不卡中文字幕,国产不卡AaV视频一区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>大鼠正常細(xì)胞>CRL-1589 IEC-18 大鼠回腸細(xì)胞

CRL-1589 IEC-18 大鼠回腸細(xì)胞

簡(jiǎn)要描述:CRL-1589 IEC-18 大鼠回腸細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-07-25
  • 訪  問  量:2273

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1589 IEC-18 大鼠回腸細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

CRL-1589 IEC-18 大鼠回腸細(xì)胞 的詳細(xì)介紹


ATCC® Number:CRL-1589™  Price:$329.00
Designations:IEC-18

Depositors:A Quaroni

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:epithelial


Source:Organ: ileum
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:22491: Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453

26096: Quaroni A, Isselbacher KJ. Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12- dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67: 1353-1362, 1981. PubMed: 6273638





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
在线性爱欧美| 日本色图亚洲| 中文不卡日韩人妻有码| 日韩动态视频久久| 夫妻原创自拍 av xyt| 中文无码av在线播放| 粉嫩主播在线| 欧美国产精品1区2区3区| 乌兰察布市| 手机成人亚洲视频在线播放| 精品-区二区三区| 丰满少妇骚逼视频| 色鸭窝78| 舔舔干天天天天爽| 淫淫少妇久久久久久| 亚洲欧美久久精品| 日韩人妻五区| 虎林市| 午夜福利激情视频免费入口| 日韩 欧美p片内射在线| 欧美三区嗯嗯啊啊啊| 色播5月 传媒| 久久久精品中文字幕无码麻豆发布| 日韩精品一区二区亚洲| 粉嫩AV丝袜一区| 欧美 日本 精品 二区| 久久久国产精品盗摄| 黄片免费一区二区| 久久久精品操| 91视频天天干| 91尤物日韩精品码区| 久久久精品相信色| 91制片厂麻豆美女特区毛片| 91大尺度国模在线| 九九久久亚洲线精品一区| 精品少妇一=三区| 欧美一区二区老妪视频在线播放| 黄美女网站99热麻豆| 狠狠狠干视频狠狠| 欧美综合图片久久久| 东南亚人妻偷拍激情视频|